课题组张国晟同学于《Environmental Research》期刊上发表了题为《Detection and various environmental factors of antibiotic resistance gene horizontal transfer》的论文。
水环境中的抗生素抗性污染日益严重，耐药细菌（ARB）和抗性基因（ARGs）也引起了研究人员的关注。ARGs在水环境中的水平转移被认为是自然环境中细菌获得耐药性的主要来源之一。水平基因转移（HGT）主要包括接合、自然转化和转导，其中接合转移被研究得最多。多项研究表明，有许多环境因子可能影响ARGs在水环境中的水平转移，例如纳米材料、各种氧化剂和光等；然而，对于ARGs水平转移的检测及各种环境因子对HGT的影响，目前还缺乏系统全面的综述。因此，本论文介绍了三种HGT模式，分析了现有HGT检测方法的优缺点，总结了各种因子对ARGs水平转移的影响和机理，并重点讨论了相似因素造成不同影响的可能原因，最后讨论了有关水环境中ARGs水平转移现有研究的不足之处和未来的研究方向。
Abstract: Bacterial antibiotic resistance in water environments is becoming increasingly severe, and new antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have also attracted the attention of researchers. The horizontal transfer of ARGs in water environments is considered one of the main sources of bacterial resistance in the natural environment. Horizontal gene transfer (HGT) mainly includes conjugation, natural transformation, and transduction, and conjugation has been investigated most. Several studies have shown that there are a large number of environmental factors that might affect the horizontal transfer of ARGs in water environments, such as nanomaterials, various oxidants, and light; however, there is still a lack of systematic and comprehensive reviews on the detection and the effects of the influence factors of on ARG horizontal transfer. Therefore, this study introduced three HGT modes, analysed the advantages and disadvantages of current methods for monitoring HGT, and then summarised the influence and mechanism of various factors on ARG horizontal transfer, and the possible reasons for the different effects caused by similar factors were mainly critically discussed. Finally, existing research deficiencies and future research directions of ARG horizontal transfer in water environments were discussed.
Keywords: Antibiotic resistant bacteria; Antibiotic resistance genes; Conjugation; Natural transformation; Transduction.
【文章链接】https://www.sciencedirect.com/science/article/pii/S0013935122005941
